CCDC39 Polyclonal antibody

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  WB analysis using 29817-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29817-1-AP (CCDC39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 29817-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CCDC39 antibody (29817-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CCDC39 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 29817-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29817-1-AP targets CCDC39 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC39 fusion protein Ag31067
• Full Name: coiled-coil domain containing 39
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_181426
• Gene Symbol: CCDC39
• Gene ID (NCBI): 339829
• RRID: AB_3086172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UFE4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC39 (Coiled-coil domain-containing protein 39) is an axonemal protein whose absence results in failure to correctly assemble DNALI1-containing inner dynein arm complexes, the DRC (dynein regulatory complex) and the radial spokes, thereby causing axonemal disorganization and dyskinetic beating. Alternatively, CCDC39 could participate in the transport of the inner dynein arms and DRC (PMID: 21131972). Its calculated molecular weight and observed molecular weight are both 110kDa.