Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 83992-1-RR
WB result of CCDC22 antibody (83992-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CCDC22 is a ubiquitously expressed coiled-coil domain protein. CCDC22 functions in the regulation of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells) by interacting with COMMD (copper metabolism Murr1 domain-containing) proteins. ]
Various lysates were subjected to SDS PAGE followed by western blot with 83992-1-RR (CCDC22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 83992-1-RR
WB result of CCDC22 antibody (83992-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC22 transfected HepG2 cells.
AFFINITY Figures
Affinity and Kinetic Characterization of 83992-1-RR
Biolayer interferometry (BLl) kinetic assays of 83992-1-RR against Human CCDC22 were performed. The affinity constant is 2.71 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CCDC22 Recombinant antibody
Catalog Number
83992-1-RR
Citations
-
Dilutions
WB : 1:2000-1:10000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
