CCDC18 Polyclonal antibody

CCDC18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30024-1-AP

Synonyms

CCDC18, dJ717I23.1, NY SAR 41, RP4 717I23.1, Sarcoma antigen NY SAR 24

---

Validation Data Gallery

• 

  WB analysis using 30024-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30024-1-AP (CCDC18 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat colon using 30024-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 30024-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 30024-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC18 antibody (30024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A431 cells, Daudi cells, HL-60 cells, HeLa cells
• Positive IHC detected in: rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30024-1-AP targets CCDC18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC18 fusion protein Ag32681
• Full Name: coiled-coil domain containing 18
• Observed Molecular Weight: 151 kDa
• GenBank Accession Number: BC156745
• Gene Symbol: CCDC18
• Gene ID (NCBI): 343099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC18 (Coiled-coil domain-containing protein 18) is a Protein that in humans is encoded by the CCDC18 gene. CCDC18 has 2 isoforms with the MW of 168 kDa and 151 kDa.