CCDC167 Polyclonal antibody

• 

  WB analysis using 31922-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31922-1-AP (CCDC167 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 31922-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CCDC167 antibody (31922-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CCDC167 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31922-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Transmembrane And Coiled-Coil Domain-Containing Protein C6orf129, HSPC265, Coiled-coil domain-containing protein 167, Coiled-Coil Domain Containing 167, Chromosome 6 Open Reading Frame 129

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HuH-7 cells, LNCaP cells, Raji cells, Ramos cells
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31922-1-AP targets CCDC167 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC167 fusion protein Ag37080
• Full Name: chromosome 6 open reading frame 129
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC108655
• Gene Symbol: C6orf129
• Gene ID (NCBI): 154467
• RRID: AB_3670146
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P0B6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Coiled-coil domain (CCD) constituents are alpha-helix motifs expressed in different types of proteins, which can function in various biological processes, including cell proliferation, migration, and signal transduction (PMID: 9171830; 17428801). The coiled-coil domain containing 167 (CCDC167, also known as HSPC265 and C6orf129) is a membrane protein highly expressed in the lymph node. It is associated with immune function, which could be a therapeutic target for breast cancer and asthma (PMID: 33461170; 31821602).