Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 84106-3-RR
WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells.
WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells.
FC experiment of U2OS using 84106-3-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 84106-3-RR
Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 84106-3-RR (CCDC134 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 84106-3-RR
WB result of CCDC134 antibody (84106-3-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC134 transfected HEK-293 cells.
FC (INTRA) Figures
FC experiment of U2OS using 84106-3-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug CCDC134 Recombinant antibody (84106-3-RR, Clone:241212F11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 84106-3-RR
Biolayer interferometry (BLl) kinetic assays of 84106-3-RR against Human CCDC134 were performed. The affinity constant is 1.69 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
CCDC134 Recombinant antibody
Catalog Number
84106-3-RR
Citations
-
Dilutions
WB : 1:2000-1:10000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
