CCDC111/PRIMPOL Polyclonal antibody

• Featured Product
• KD/KO Validated

CCDC111/PRIMPOL Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 29824-1-AP

Synonyms

CCDC111, FLJ33167, hPrimpol1, PRIMPOL

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Validation Data Gallery

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  WB analysis using 29824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 29824-1-AP

  WB result of CCDC111/PRIMPOL antibody (29824-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCDC111/PRIMPOL transfected HepG2 cells.

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  IHC staining of human kidney using 29824-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29824-1-AP (CCDC111/PRIMPOL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 29824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC111 antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 29824-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CCDC111 antibody (29824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Product Information

29824-1-AP targets CCDC111/PRIMPOL in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCDC111/PRIMPOL fusion protein Ag31478
• Full Name: coiled-coil domain containing 111
• Observed molecular weight: 64 kDa
• GenBank accession number: BC064600
• Gene symbol: CCDC111
• Gene ID (NCBI): 201973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hasan (Verified Customer) (01-31-2023)

WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa cells/ different clones

CCDC111/PRIMPOL Antibody Western Blot validation (1:2000 dilution) in HeLa cells/ different clones (Cat no:29824-1-AP)