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CCDC111/PRIMPOL Polyclonal antibody

CCDC111/PRIMPOL Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29824-1-AP

Synonyms

CCDC111, FLJ33167, hPrimpol1, PRIMPOL



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29824-1-AP targets CCDC111/PRIMPOL in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC111/PRIMPOL fusion protein Ag31478
Full Name coiled-coil domain containing 111
Observed molecular weight64 kDa
GenBank accession numberBC064600
Gene symbol CCDC111
Gene ID (NCBI) 201973
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).

Protocols

Product Specific Protocols
WB protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IF protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols