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  • KD/KO Validated

CCDC111/PRIMPOL Polyclonal antibody

CCDC111/PRIMPOL Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human and More (1)





Cat no : 29824-1-AP


CCDC111, FLJ33167, hPrimpol1, PRIMPOL

Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

29824-1-AP targets CCDC111/PRIMPOL in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCDC111/PRIMPOL fusion protein Ag31478
Full Name coiled-coil domain containing 111
Observed Molecular Weight64 kDa
GenBank Accession NumberBC064600
Gene Symbol CCDC111
Gene ID (NCBI) 201973
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCDC111, also known as PRIMPOL, FLJ33167, which contains the active-site motifs of AEP primases and a putative Zn finger similar to that of herpesvirus UL52 primase and other AEP-like enzymes. CCDC111 is required to facilitate mitochondrial and nuclear replication fork progression by initiating de novo DNA synthesis using dNTPs and acting as an error-prone DNA polymerase able to bypass certain DNA lesions (PMID: 24126761, PMID: 24207056). In addition to its role in DNA damage response, also required to maintain efficient nuclear and mitochondrial DNA replication in unperturbed cells (Uniprot, PMID: 30715459).


Product Specific Protocols
WB protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IHC protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
IF protocol for CCDC111/PRIMPOL antibody 29824-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

An ATR-PrimPol pathway confers tolerance to oncogenic KRAS-induced and heterochromatin-associated replication stress

Authors - Taichi Igarashi

Nat Commun

Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases

Authors - Anastasia Hale

Int J Mol Sci

CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin

Authors - Nicole L Batenburg

Nucleic Acids Res

CSB and SMARCAL1 compete for RPA32 at stalled forks and differentially control the fate of stalled forks in BRCA2-deficient cells

Authors - Nicole L Batenburg

Nucleic Acids Res

The levels of p53 govern the hierarchy of DNA damage tolerance pathway usage

Authors - Bryan A Castaño


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hasan (Verified Customer) (01-31-2023)

WB analysis of PrimPol in whole lysate of different clones of HeLa cells. Blocking was done for 1h at room temperature with 5% skimmed milk. The primary Ab was used with 1:2000 dilution overnight at 4 degrees.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HeLa cells/ different clones
CCDC111/PRIMPOL Antibody Western Blot validation (1:2000 dilution) in HeLa cells/ different clones (Cat no:29824-1-AP)