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ACKR2 Polyclonal antibody

ACKR2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11386-1-AP

Synonyms

CCBP2, CCR9, CCR10, C-C chemokine receptor D6, C C chemokine receptor D6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ACKR2 Polyclonal antibody (11386-1-AP)

    IHC staining of human tonsillitis using 11386-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11386-1-AP (ACKR2 Antibody) at dilution of 1:1200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ACKR2 Polyclonal antibody (11386-1-AP)

    IHC staining of human tonsillitis using 11386-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11386-1-AP (ACKR2 Antibody) at dilution of 1:1200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using ACKR2 Polyclonal antibody (11386-1-AP)

    IHC staining of human tonsillitis using 11386-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11386-1-AP (ACKR2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11386-1-AP targets ACKR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACKR2 fusion protein Ag1941
Full Name chemokine binding protein 2
Calculated Molecular Weight 43 kDa
GenBank Accession NumberBC008816
Gene Symbol ACKR2
Gene ID (NCBI) 1238
RRIDAB_2877760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACKR2, also named as CCBP2 or chemokine receptor D6, is a heptahelical membrane protein expressed by lymphatic endothelial cells, binding with high affinity to multiple proinflammatory CC chemokines.

Protocols

Product Specific Protocols
IHC protocol for ACKR2 antibody 11386-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Transl Res

The mechanism of chemokine receptor ACKR2 in cognitive impairment in young rats with traumatic brain injury

Authors - Lijuan Quan
View Article