CCBL1 Polyclonal antibody

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  WB analysis using 30296-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30296-1-AP (CCBL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 30296-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CCBL1 antibody (30296-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CCBL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30296-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

CCBL1, FLJ95217, Glutamine transaminase K, GTK, KAT1, KATI, Kynurenine aminotransferase I

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, U-87 MG cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30296-1-AP targets CCBL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CCBL1 fusion protein Ag32316
• Full Name: cysteine conjugate-beta lyase, cytoplasmic
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC033685
• Gene Symbol: CCBL1
• Gene ID (NCBI): 883
• RRID: AB_2935538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16773
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCBL1 encodes kynurenine aminotransferase 1 (KAT1), a key enzyme involved in kynurenine pathway. CCBL1 catalyzes the production of kynurenic acid, a powerful endogenous excitatory amino acid receptor antagonist that is widely regarded as the potent neuroprotective agent.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (08-30-2024)

Works well in WB with mouse liver lysate. Background is clean. The signal is not very strong.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver

CCBL1 Antibody Western Blot validation (1:500 dilution) in Mouse liver (Cat no:30296-1-AP)