CC2D1B Polyclonal antibody

• Featured Product
• KD/KO Validated

CC2D1B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA, IF

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 20774-1-AP

Synonyms

CC2D1B, Freud-2, KIAA1836, LGD1

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Validation Data Gallery

• 

  WB analysis of mouse brain using 20774-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 20774-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20774-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 20774-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 20774-1-AP (CC2D1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20774-1-AP

  IP result of anti-CC2D1B (IP:20774-1-AP, 4ug; Detection:20774-1-AP 1:500) with mouse brain tissue lysate 2560ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, human placenta tissue, mouse kidney tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Product Information

The immunogen of 20774-1-AP is CC2D1B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CC2D1B fusion protein Ag14767
• Full Name: coiled-coil and C2 domain containing 1B
• Calculated Molecular Weight: 858 aa, 94 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC007912
• Gene Symbol: CC2D1B
• Gene ID (NCBI): 200014
• RRID: AB_10693627
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CC2D1B/Freud2 and CC2D1A/Freud1 are two homologs belonging to the evolutionary conserved Lgd protein family that is involved in endosomal trafficking. CC2D1B has been identified as the novel repressor of postsynaptic 5-HT1A expression. The predicted molecular weight of CC2D1B is 89 kDa, but the observed molecular weight is around 120 kDa, which may be caused by post-translational modification. This antibody is specific to CC2D1B, which has been confirmed by siRNA test. (19423080 , 2672064)

Publications

Species	Application	Title
mouse	WB,IF	PLoS Genet; The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.; Authors - Nadja Drusenheimer ; KO Validated
mouse	WB	Front Mol Neurosci; Cc2d1b Contributes to the Regulation of Developmental Myelination in the Central Nervous System.; Authors - Jenica Acheta ; KO Validated
rat	WB,IF	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie ; KD Validated
human	IF	Biol Pharm Bull; Icariin regulates EMT and stem cell-like character in breast cancer through modulating lncRNA NEAT1/TGFβ/SMAD2 signaling pathway; Authors - Bo Song
human	WB	Nat Commun; Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef; Authors - Caterina Prelli Bozzo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Abigail (Verified Customer) (09-16-2019)

It worked well for us

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: Murine Hippocampus and cortex