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CC2D1A Monoclonal antibody, PBS Only

CC2D1A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C11G2

Cat no : 68334-1-PBS

Synonyms

CC2D1A, FLJ20241, FLJ41160, FREUD 1, Freud 1/Aki1, MRT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CC2D1A Monoclonal antibody (68334-1-Ig)

    WB analysis using 68334-1-Ig (same clone as 68334-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68334-1-Ig (CC2D1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CC2D1A Monoclonal antibody (68334-1-Ig)

    IHC staining of mouse testis using 68334-1-Ig (same clone as 68334-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68334-1-Ig (CC2D1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CC2D1A Monoclonal antibody (68334-1-Ig)

    IF Staining of HeLa using 68334-1-Ig (same clone as 68334-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CC2D1A antibody (68334-1-Ig, Clone: 1C11G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68334-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68334-1-PBS targets CC2D1A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CC2D1A fusion protein Ag10363
Full Name coiled-coil and C2 domain containing 1A
Calculated Molecular Weight 951 aa, 104 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC064981
Gene Symbol CC2D1A
Gene ID (NCBI) 54862
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.