CC2D1A Polyclonal antibody

• Featured Product
• KD/KO Validated

CC2D1A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16816-1-AP

Synonyms

FREUD 1, FRE under dual repression-binding protein 1, Five prime repressor element under dual repression-binding protein 1, Coiled-coil and C2 domain-containing protein 1A, Akt kinase-interacting protein 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 16816-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16816-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16816-1-AP

  IP result of anti-CC2D1A (IP:16816-1-AP, 4ug; Detection:16816-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16816-1-AP

  Immunofluorescent analysis of HepG2 cells, using CC2D1A antibody 16816-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human testis tissue, human brain tissue, human liver tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

16816-1-AP targets CC2D1A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CC2D1A fusion protein Ag10355
• Full Name: coiled-coil and C2 domain containing 1A
• Calculated Molecular Weight: 951 aa, 104 kDa
• Observed Molecular Weight: 130 kDa, 104 kDa
• GenBank Accession Number: BC064981
• Gene Symbol: CC2D1A
• Gene ID (NCBI): 54862
• RRID: AB_2275394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

Publications

Species	Application	Title
rat	WB	Anesthesiology; Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats.; Authors - Song Zhang
mouse	WB	J Biol Chem; TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.; Authors - Chen Kuan-Ru KR ; KD Validated
mouse	WB	Neurotherapeutics; Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice.; Authors - Cheng-Yi Yang
mouse	WB	J Neurosci; Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.; Authors - Cheng-Yi Yang