CBX5 Polyclonal antibody

• Featured Product
• KD/KO Validated

CBX5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, chIP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (2)

Cat no : 11831-1-AP

Synonyms

Antigen p25, CBX5, Chromobox protein homolog 5, HP1, HP1 alpha, HP1A

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Validation Data Gallery

• 

  WB analysis of HeLa using 11831-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11831-1-AP

  WB result of CBX5 antibody (11831-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CBX5 transfected K-562 cells.

• 

  WB analysis of MCF-7 using 11831-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11831-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11831-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11831-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11831-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11831-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11831-1-AP (CBX5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11831-1-AP

  IP result of anti-CBX5 (IP:11831-1-AP, 4ug; Detection:11831-1-AP 1:500) with HEK-293 cells lysate 1120ug.

• 

  IHC staining of human lung cancer using 11831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11831-1-AP (CBX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11831-1-AP (CBX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human kidney tissue, K-562 cells, A431 cells, MCF-7 cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Product Information

11831-1-AP targets CBX5 in WB, IP, IHC, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBX5 fusion protein Ag2403
• Full Name: chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• Calculated Molecular Weight: 191 aa, 22 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC006821
• Gene Symbol: CBX5
• Gene ID (NCBI): 23468
• RRID: AB_2071356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromobox protein homolog 5 (CBX5), also named heterochromatin protein 1 alpha (HP1a), is a highly conserved nonhistone protein involved in heterochromatin formation and gene silencing in different species including humans.HP1a is a Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). It may interact with lamin-Breceptor. HP1a is involved in the formation of functional kinetochore through interaction with MIS12complex proteins. Phosphorylation of HP1 and LBR during interphase mitosis may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle.The HP1a was expressed in nucleus and associates specifically with chromatin during metaphase and anaphase.Recent studies have shown that HP1a is present at many euchromatic sites and positively regulates euchromatic gene expression through RNA transcript association and interaction with hnRNPs in Drosophila (19798443). This antibody is a rabbit polyclonal antibody raised against a full-length human HP1A protein ,and can reacts with the 28kd HP1A protein.

Publications

Species	Application	Title
mouse	WB,IP	J Exp Med; ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.; Authors - Longfei Ma
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB,chIP	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang
human	WB	Asian Pac J Cancer Prev; Triptolide Promotes Senescence of Prostate Cancer Cells Through Histone Methylation and Heterochromatin Formation; Authors - Ousman Tamgue
human	WB	Microbiol Spectr; Porphyromonas gingivalis Outer Membrane Vesicles Promote Apoptosis via msRNA-Regulated DNA Methylation in Periodontitis; Authors - Ruyi Fan ; KD Validated
human	WB,IP	Oncogene; ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression; Authors - Lifang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-13-2024)

Works well to detect CBX5 in total and nuclear extracts of HeLa cells by WB (1/1000 dilution - Overnight incubation at 4°C).

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa

FH

WEI (Verified Customer) (05-11-2022)

Specific bands at predicted size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart