CBX3 Monoclonal antibody, PBS Only

CBX3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5A3

Cat no : 66446-1-PBS

Synonyms

CBX3, Chromobox protein homolog 3, HECH, HP1 GAMMA, HP1Hs gamma, Modifier 2 protein

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 66446-1-Ig (same clone as 66446-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66446-1-Ig (same clone as 66446-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66446-1-Ig (same clone as 66446-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66446-1-Ig (same clone as 66446-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66446-1-Ig (same clone as 66446-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66446-1-Ig (CBX3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66446-1-Ig (same clone as 66446-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBX3 antibody (66446-1-Ig, Clone: 1A5A3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66446-1-PBS in a different storage buffer formulation.

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Product Information

66446-1-PBS targets CBX3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CBX3 fusion protein Ag19112
• Full Name: chromobox homolog 3 (HP1 gamma homolog, Drosophila)
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000954
• Gene Symbol: CBX3
• Gene ID (NCBI): 11335
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The family of Heterochromatin protein 1 (HP1) proteins is a family of highly conserved heterochromatin-associated non-histone chromosomal proteins , which has important functions in nucleus. These functions include gene activation or repression, regulation of binding of cohesion complexes to centromere, sequestration of genes to nuclear periphery, and heterochromatin formation and propagation. Much evidence shows that HP1 proteins interact with numerous proteins including methylated histones, histone methyltransferases and so on. CBX3 is one of the paralogues of HP1 proteins