CBR1 Recombinant antibody

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  WB analysis using 83736-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83736-4-RR (CBR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83736-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (83736-4-RR, Clone: 240707G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83736-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83736-4-RR against Human CBR1 were performed. The affinity constant is 13.7 nM.

CBR1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83736-4-RR

Clone No.240707G1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 240707G1, 20-beta-hydroxysteroid dehydrogenase

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, HEK-293T cells, MCF-7 cells, SKOV-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83736-4-RR targets CBR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBR1 fusion protein Ag34830
• Full Name: carbonyl reductase 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC002511
• Gene Symbol: CBR1
• Gene ID (NCBI): 873
• RRID: AB_3671336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P16152
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBR1 belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics.