CBR1 Polyclonal antibody

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  WB analysis using 31279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31279-1-AP (CBR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 31279-1-AP

  IP result of anti-CBR1 (IP:31279-1-AP, 4ug; Detection:31279-1-AP 1:5000) with HeLa cells lysate 980 ug.

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  IF Staining of HeLa using 31279-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CBR1 antibody (31279-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CBR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 31279-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

EC:1.1.1.189, EC:1.1.1.184, carbonyl reductase 1, Alcohol dehydrogenase [NAD(P)+] CBR1, 20-beta-hydroxysteroid dehydrogenase

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Tested Applications

• Positive WB detected in: HeLa cells, H9C2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31279-1-AP targets CBR1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBR1 fusion protein Ag34830
• Full Name: carbonyl reductase 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC002511
• Gene Symbol: CBR1
• Gene ID (NCBI): 873
• RRID: AB_3669926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P16152
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CBR1 belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics.