Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, human brain tissue, Jurkat cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10349-1-AP targets NCBP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NCBP1 fusion protein Ag0496|
|Full Name||nuclear cap binding protein subunit 1, 80kDa|
|Calculated molecular weight||92 kDa|
|Observed molecular weight||80 kDa|
|GenBank accession number||BC001450|
|Gene ID (NCBI)||4686|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.
RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.
RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits
Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.
SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.
Nucleic Acids Res
SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.
AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.