NCBP1/CBP80 Polyclonal antibody

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  WB analysis using 10349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10349-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10349-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10349-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10349-1-AP (NCBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10349-1-AP

  IP result of anti-NCBP1 (IP:10349-1-AP, 4ug; Detection:10349-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human lung cancer using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10349-1-AP (NCBP1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10349-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10349-1-AP (NCBP1 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

NCBP1/CBP80 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10349-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

NCBP1, 80 kDa nuclear cap-binding protein, CBP80, NCBP, NCBP 80 kDa subunit

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 3 publications below

Product Information

10349-1-AP targets NCBP1/CBP80 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0496

  Product name: Recombinant human CBP80 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 46-350 aa of BC001450

  Sequence: LESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTADKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYH

• Full Name: nuclear cap binding protein subunit 1, 80kDa
• Calculated Molecular Weight: 92 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC001450
• Gene Symbol: NCBP1
• Gene ID (NCBI): 4686
• RRID: AB_2150127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q09161
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear cap binding protein subunit 1, 80kDa (NCBP1, synonyms: NCBP, CBP80) is a subunit of a cap-binding protein complex (CBC) that consists of a CBP20 subunit, which binds the cap, and a NCBP1 subunit, which ensures high-affinity cap binding. The CBC complex may play a role in pre-mRNA recognition and splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.; Authors - Fustin Jean-Michel JM ; KD Validated
mouse	WB,IP	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Mol Cell; Efficiency of the pioneer round of translation affects the cellular site of nonsense-mediated mRNA decay.; Authors - Hanae Sato
human	WB,IP	Genes Dev; SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.; Authors - Yamashita Akio A
human	WB	Nucleic Acids Res; SUMOylation of the m6A-RNA methyltransferase METTL3 modulates its function.; Authors - Yuzhang Du
human	WB	Sci Signal; AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.; Authors - Izumi Natsuko N