CBLL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

CBLL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 21179-1-AP

Synonyms

HAKAI, EC:2.3.2.27, E3 ubiquitin-protein ligase Hakai, c-Cbl-like protein 1, Casitas B-lineage lymphoma-transforming sequence-like protein 1

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Validation Data Gallery

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  WB analysis using 21179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21179-1-AP (CBLL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 21179-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21179-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 21179-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21179-1-AP (CBLL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21179-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CBLL1 antibody (21179-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

21179-1-AP targets CBLL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CBLL1 fusion protein Ag15463
• Full Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1
• Calculated Molecular Weight: 491 aa, 55 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC027460
• Gene Symbol: CBLL1
• Gene ID (NCBI): 79872
• RRID: AB_10732814
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cbl proto‐oncogene E3 ubiquitin protein ligase‐like 1, RNF188, Hakai (CBLL1) is an evolutionarily conserved E3 ubiquitin ligase containing a RING‐finger domain. CBLL1 contains a typical RING‐finger, short pTyr‐binding domain, and proline‐rich domain. CBLL1 expression is upregulated in human colon and gastric cancer tissues, and CBLL1 has been reported to induce anchorage‐dependent cell growth. CBLL1 immunostaining was observed in both the nuclei and cytoplasm of cancer cells. (PMID: 31124298, PMID: 31646569, PMID: 21191016)

Publications

Species	Application	Title
human	IF	J Ginseng Res; Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop; Authors - Chunmei Hu
human	WB	Mol Biol Cell; Minus end-directed motor KIFC3 suppresses E-cadherin degradation by recruiting USP47 to adherens junctions.; Authors - Kyoko Sako-Kubota ; KD Validated
human	WB,IF	Cell Signal; Hakai, an E3-ligase for E-cadherin, stabilizes δ-catenin through Src kinase.; Authors - Hridaya Shrestha
human	WB	Med Oncol; Celastrol inhibits the migration and invasion and enhances the anti-cancer effects of docetaxel in human triple-negative breast cancer cells; Authors - Zi Liu ; KD Validated
		Int J Mol Med; PMID: 29786107; Authors - Zi Liu
mouse	WB	Front Genet; Identification and experimental validation of key m6A modification regulators as potential biomarkers of osteoporosis; Authors - Yanchun Qiao