Calcium Sensing Receptor Polyclonal antibody

• Featured Product
• KD/KO Validated

Calcium Sensing Receptor Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 19125-1-AP

Synonyms

Calcium Sensing Receptor, CAR, CASR, FHH, FIH, GPRC2A, HHC, HHC1, NSHPT, PCAR1

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Validation Data Gallery

• 

  WB analysis using 19125-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 19125-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19125-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 19125-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 19125-1-AP (Calcium Sensing Receptor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, COLO 320 cells, HL-60 cells, Jurkat cells, mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 8 publications below
• IF: See 4 publications below

Product Information

19125-1-AP targets Calcium Sensing Receptor in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium-sensing receptor
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120-140 kDa
• GenBank Accession Number: NM_000388
• Gene Symbol: Calcium Sensing Receptor
• Gene ID (NCBI): 846
• RRID: AB_10646434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASR, also named as GPRC2A, PCAR1, belongs to the G-protein coupled receptor 3 family. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. The activity of CASR is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. It has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo). The 60-65 kDa band detected by this antibody might represent a degradation product of CASR (PMID: 15718278).

Publications

Species	Application	Title
rat	WB,IHC,IF	J Ginseng Res; Ginsenoside Rg1 attenuates mechanical stress-induced cardiac injury via calcium sensing receptor-related pathway; Authors - Mei-Li Lu
human,rat	WB,IF	Front Pharmacol; Calcium Sensing Receptor-Related Pathway Contributes to Cardiac Injury and the Mechanism of Astragaloside IV on Cardioprotection.; Authors - Meili Lu
human	WB	Phytomedicine; Panaxytriol upregulates CYP3A4 expression based on the interaction of PXR, CAR, HSP90α, and RXRα.; Authors - Lingming Zhang ; KD Validated
rat	WB	Front Pharmacol; Effects of Sodium Ferulate on Cardiac Hypertrophy Are via the CaSR-Mediated Signaling Pathway.; Authors - Panpan Chen
rat	WB	J Ethnopharmacol; Fructus Ligustri Lucidi aqueous extract promotes calcium balance and short-chain fatty acids production in ovariectomized rats.; Authors - Beibei Chen
human	WB,IHC	Acta Pharmacol Sin; Astragaloside IV attenuates myocardial ischemia/reperfusion injury in rats via inhibition of calcium-sensing receptor-mediated apoptotic signaling pathways.; Authors - Bo Yin

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