Caspase 6/p18/p11 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 6/p18/p11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 10198-1-AP

Synonyms

CASP6, Caspase6, Caspase 6, CASP-6, CASP 6

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Validation Data Gallery

• 

  WB analysis of Jurkat using 10198-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10198-1-AP (CASP6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10198-1-AP

  Staurosporine treat Jurkat cells were subjected to SDS PAGE followed by western blot with 10198-1-AP (Caspase 6/p18/p11 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10198-1-AP

  WB result of Caspase 6/p18/p11 antibody (10198-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 6/p18/p11 transfected Jurkat cells.

• 

  WB analysis of mouse brain using 10198-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10198-1-AP (CASP6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10198-1-AP (CASP6 antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human skin using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10198-1-AP (CASP6 antibody) at dilution of 1:500 (under 40x lens).

• 

  IHC staining of human lung cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 10198-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10198-1-AP (CASP6 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, Jurkat cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human prostate cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IF: See 1 publications below

Product Information

10198-1-AP targets Caspase 6/p18/p11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 6/p18/p11 fusion protein Ag0257
• Full Name: caspase 6, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 33 kDa, 22 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC000305
• Gene Symbol: Caspase 6
• Gene ID (NCBI): 839
• RRID: AB_2259380
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase-6 belongs to caspase family of cysteinyl-aspartate specific proteases.Precursor of CASP6 produces two subunits, large (18kDa) and small (16kDa) that dimerize. It cleaves poly(ADP-ribose) polymerase, as well as lamins and is involved in the activation cascade of caspases responsible for apoptosis execution. Researches showed that CASP6 could be an early instigator of neuronal dysfunction and regulates B cell activation and differentiation into plasma cells by modifying cell cycle entry. IRAK3 is an important target for CASP6. It can reveal five bands of 28, 32, 36, 49, and 64 kDa in human neurons and fetal brain in western blot, the 32 and 28 kDa bands represent procaspase-6 and pro-arm caspase-6. Procaspase-6 is more abundant than pro-arm caspase-6 in adult tissue, whereas pro-arm caspase-6 is more abundant than pro-caspase-6 in fetal brain and cultured neurons. The higher molecular mass bands at 49 and 64 kDa likely represent dimers of p28 and p32.(PMID:10438520). In rat testis, it can be detected two bands of 34 kDa and 12 kDa or 14 kDa(PMID:12538628).

Publications

Species	Application	Title
rat	WB,IF	J Pharm Anal; Cornus officinalis with high pressure wine steaming enhanced anti-hepatic fibrosis: Possible through SIRT3-AMPK axis; Authors - Xin Han
mouse	WB	Transl Stroke Res; Melatonin Offers Dual-Phase Protection to Brain Vessel Endothelial Cells in Prolonged Cerebral Ischemia-Recanalization Through Ameliorating ER Stress and Resolving Refractory Stress Granule; Authors - Danli Lu
human	WB	Chem Biol Interact; O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.; Authors - Han Liao
human	WB	Biotechnol Bioeng; Generation of apoptosis-resistant HEK293 cells with CRISPR/Cas mediated quadruple gene knockout for improved protein and virus production.; Authors - Weifeng Zhang ; KO Validated
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB	Front Pharmacol; Astragalin Inhibits the Proliferation and Migration of Human Colon Cancer HCT116 Cells by Regulating the NF-κB Signaling Pathway.; Authors - Min Yang