Caspase 4/p20/p10 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 4/p20/p10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 11856-1-AP

Synonyms

CASP4, Caspase4, Caspase-4, Caspase 4, CASP-4

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Validation Data Gallery

• 

  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11856-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11856-1-AP (Caspase 4/p20/p10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 11856-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11856-1-AP (CASP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 11856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11856-1-AP (CASP4 antibody) at dilution of 1:50 (under 25x lens).

• 

  IF Staining of HepG2 using 11856-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Caspase 4/p20/p10 antibody (11856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, THP-1 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below

Product Information

11856-1-AP targets Caspase 4/p20/p10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 4/p20/p10 fusion protein Ag2429
• Full Name: caspase 4, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 377 aa, 43 kDa
• Observed Molecular Weight: 43-48 kDa
• GenBank Accession Number: BC017839
• Gene Symbol: Caspase 4
• Gene ID (NCBI): 837
• RRID: AB_2070053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Publications

Species	Application	Title
human	IHC,WB	Front Immunol; Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma; Authors - Liangmin Fu ; KD Validated
human	WB	Front Microbiol; Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.; Authors - Pei-Hui Ding
human	WB	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
human	WB	Oral Dis; Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway; Authors - Bing-Jing Sun
human	WB	Chem Res Toxicol; Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.; Authors - Xiaomin Xia
human	WB	Int J Mol Sci; PMID: 27827850; Authors - Wei-Ye Shi