Caspase 2/p32/p18 Polyclonal antibody

Caspase 2/p32/p18 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, RIP, ELISA, IF

Conjugate

Unconjugated

Publications

8

Cat no : 10436-1-AP

Synonyms

CASP 2, CASP2, Caspase 2, Caspase 2/p18, Caspase 2/p32/p18, Caspase2, ICH 1L, ICH 1L/1S, ICH1, NEDD 2, NEDD2, Protease ICH 1

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Validation Data Gallery

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  WB analysis of Jurkat using 10436-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (Caspase 2/p18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10436-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10436-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10436-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10436-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10436-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10436-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10436-1-AP (CASP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10436-1-AP

  IP result of anti-Caspase 2/p32/p18 (IP:10436-1-AP, 4ug; Detection:10436-1-AP 1:2000) with HeLa cells lysate 3000 ug.

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10436-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10436-1-AP (Caspase 2/p18 antibody at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Staurosporine treated Jurkat cells, HEK-293 cells, HeLa cells, HL-60 cells, Jurkat cells, mouse kidney tissue, mouse liver tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below
• RIP: See 1 publications below

Product Information

10436-1-AP targets Caspase 2/p32/p18 in WB, IHC, IP, RIP, ELISA, IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Caspase 2/p32/p18 fusion protein Ag0694
• Full Name: caspase 2, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 48-51 kDa, 32 kDa, 18kDa
• GenBank Accession Number: BC002427
• Gene Symbol: Caspase 2
• Gene ID (NCBI): 835
• RRID: AB_2228450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASP2(Caspase-2) is also named as ICH1, NEDD2 and belongs to the peptidase C14A family.It is involved in the activation cascade of caspases responsible for apoptosis execution and might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival.It has 2 isoforms produced by alternative splicing and can exists almost as a dimer in solution(PMID:15865942).

Publications

Species	Application	Title
human	WB, IF	Front Microbiol; Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.; Authors - Bei Wang
		Front Pharmacol; PMID: 32210806; Authors - Wen Su
rabbit	RIP	Life Sci; Timolol induces necroptosis, apoptosis and senescence concentration-dependently in rabbit Limbal stem cells in vitro.; Authors - Guo-Jian Jiang
human	ELISA	Toxicol In Vitro; PMID: 32320760; Authors - Ting Yang
mouse	WB	Toxicon; Involvement of caspase in patulin-induced hepatotoxicity in vitro and in vivo.; Authors - Baigang Zhang
		Chem Biol Interact; Different concentrations of betaxolol switch cell fate between necroptosis, apoptosis, and senescence in human corneal stromal cells; Authors - Jing-Yu Sun