Caspase 12 Polyclonal antibody

• Featured Product
• KD/KO Validated

Caspase 12 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

100

Cat no : 55238-1-AP

Synonyms

CASP 12, CASP12, CASP12P1, Caspase 12, caspase 12 (gene/pseudogene), Caspase12, Inactive caspase 12

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Validation Data Gallery

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  WB analysis of HEK-293 using 55238-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells using 55238-1-AP

  WB result of 55238-1-AP (CASP12 antibody) with various lysates.

• 

  WB analysis of HepG2 using 55238-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55238-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 55238-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55238-1-AP (CASP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55238-1-AP

  IP result of anti-Caspase 12 (IP:55238-1-AP, 4ug; Detection:55238-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 55238-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55238-1-AP (Caspase 12 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55238-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55238-1-AP (Caspase 12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, multi-cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate hyperplasia tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 96 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Product Information

55238-1-AP targets Caspase 12 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: caspase 12 (gene/pseudogene)
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 36-42 kDa, 50 kDa
• GenBank Accession Number: NR_000035
• Gene Symbol: Caspase 12
• Gene ID (NCBI): 120329
• RRID: AB_10892670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. Caspase-12 is located in the endoplasmic reticulum (ER). It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels. Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50 kDa) and its cleaved product (40-44 kDa). This antibody does not cross-react with other caspases.

Publications

Species	Application	Title
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB	ACS Appl Mater Interfaces; Endoplasmic Reticulum-Targeted Aggregation-Induced Emission Luminogen for Synergetic Tumor Ablation with Glibenclamide; Authors - Yifan Wu
	WB	J Hazard Mater; Perinatal low-dose PBDE-47 exposure hampered thyroglobulin turnover and induced thyroid cell apoptosis by triggering ER stress and lysosomal destabilization contributing to thyroid toxicity in adult female rats.; Authors - Pei Li
human	WB,IF	Theranostics; Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo.; Authors - Zhiwei Liao
human	WB	Biomed Pharmacother; A novel synthetic curcumin derivative MHMM-41 induces ROS-mediated apoptosis and migration blocking of human lung cancer cells A549.; Authors - Guang-Zhou Zhou
human	WB	Oxid Med Cell Longev; Betulinic Acid Suppresses Breast Cancer Metastasis by Targeting GRP78-Mediated Glycolysis and ER Stress Apoptotic Pathway.; Authors - Yifeng Zheng

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