CASK Polyclonal antibody

• Featured Product
• KD/KO Validated

CASK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55277-1-AP

Synonyms

CAGH39, CASK, CMG, FGS4, hCASK, LIN2, MICPCH, Protein lin 2 homolog, TNRC8

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Validation Data Gallery

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  WB analysis using 55277-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 55277-1-AP

  WB result of CASK antibody (55277-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CASK transfected A549 cells.

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  WB analysis of mouse liver using 55277-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55277-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55277-1-AP (CASK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 55277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 55277-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 55277-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 55277-1-AP (CASK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse liver tissue, A549 cells, rat brain tissue
• Positive IHC detected in: rat small intestine tissue, mouse kidney tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55277-1-AP targets CASK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 100-110 kDa, 50-55 kDa
• GenBank Accession Number: NM_003688
• Gene Symbol: CASK
• Gene ID (NCBI): 8573
• RRID: AB_10858487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CASK, also named as LIN2, belongs to the MAGUK family. It is a multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. It contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. CASK binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. It may mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Defects in CASK are the cause of mental retardation X-linked CASK-related (MRXCASK) . Defects in CASK are the cause of FG syndrome type 4 (FGS4). This antibody can recognize all the isoform of CASK.