CARS Monoclonal antibody, PBS Only

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  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67933-1-Ig (same clone as 67933-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67933-1-Ig (same clone as 67933-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67933-1-Ig (CARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67933-1-Ig (same clone as 67933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CARS antibody (67933-1-Ig, Clone: 2F7E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67933-1-PBS in a different storage buffer formulation.

CARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67933-1-PBS

Clone No.2F7E4

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

CARS, CARS1, CYSRS, Cysteine tRNA ligase, cysteinyl tRNA synthetase

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Product Information

67933-1-PBS targets CARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CARS fusion protein Ag7651
• Full Name: cysteinyl-tRNA synthetase
• Calculated Molecular Weight: 89 kDa
• Observed Molecular Weight: 70-85 kDa
• GenBank Accession Number: BC002880
• Gene Symbol: CARS
• Gene ID (NCBI): 833
• RRID: AB_2918685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49589
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.