CARM1 Recombinant antibody

• 

  WB analysis using 85146-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85146-3-RR (CARM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CARM1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85146-3-RR

Clone No.242651F10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Protein arginine N-methyltransferase 4, PRMT4, Histone-arginine methyltransferase CARM1, EC:2.1.1.319, Coactivator-associated arginine methyltransferase 1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, HCT 116 cells, MCF-7 cells, NCI-H1299 cells, NIH/3T3 cells, PC-12 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85146-3-RR targets CARM1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: coactivator-associated arginine methyltransferase 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 63-65 kDa
• GenBank Accession Number: NM_199141
• Gene Symbol: CARM1
• Gene ID (NCBI): 10498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86X55
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.