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  • KD/KO Validated

CARM1 Polyclonal antibody

CARM1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55246-1-AP

Synonyms

CARM1, PRMT4



Tested Applications

Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55246-1-AP targets CARM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name coactivator-associated arginine methyltransferase 1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 63-66 kDa, 55 kDa
GenBank Accession NumberNM_199141
Gene Symbol CARM1
Gene ID (NCBI) 10498
RRIDAB_10863291
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARM1, also named as PRMT4, belongs to the protein arginine N-methyltransferase family. It is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein. CARM1 functions as a coactivator for many nuclear receptors, such as oestrogen receptor, androgen receptor, thyroid receptor and farnesoid X-receptor. It also coactivates other transcription factors such as myocyte enhancer factor 2C (MEF2C), β-catenin, p53, nuclear factor (NF)-kB and the cAMP-responsive element-binding factor. The enzymatic activity and coactivator function of CARM1 has been found to be inactivated through phosphorylation at a conserved serine residue at mitosis stage. This antibody was generated against a synthetic peptide corresponding to a fragment of human CARM1. It is expected to specifically recognize the CRAM1. In certain type of cells, like Hela, double bands can be detected with this antibody. This may due to the additional PTM sites in cells themselves.

Protocols

Product Specific Protocols
WB protocol for CARM1 antibody 55246-1-APDownload protocol
IHC protocol for CARM1 antibody 55246-1-APDownload protocol
IF protocol for CARM1 antibody 55246-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of First-in-Class Dual PARP and EZH2 Inhibitors for Triple-Negative Breast Cancer with Wild-Type BRCA

Authors - Limei Ji
humanWB

Eur J Med Chem

Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.

Authors - Cheng Wang
mouseWB

Front Cell Dev Biol

Osteocytes Enhance Osteogenesis by Autophagy-Mediated FGF23 Secretion Under Mechanical Tension.

Authors - Huiyue Xu
humanWB,CoIP

Cancer Cell Int

CDCA4 suppresses epithelial-mesenchymal transtion (EMT) and metastasis in Non-small cell lung cancer through modulating autophagy.

Authors - Chenxin Xu
  • KD Validated
humanWB

RSC Adv

Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression

Authors - Guixin Wu
mouseIF

J Reprod Immunol

Poly(I:C) exposure during in vitro fertilization disrupts first cleavage of mouse embryos and subsequent blastocyst development.

Authors - Zhicheng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

fatemeh (Verified Customer) (07-25-2022)

western blot was done on 50 ug of cell lysate. Blot was transfered using wet transfer method (300 mA for 90 min). 5% milk was used as blocking reagent. antibody dilution was 1:1000 and incubation with primary antibody was overnight at 4C. Multiple band between 75 and 50 kDa were detected. for IF, PFA was used for fixing the cells and 0.5% triton was used for permeabilization. antibody was diluted 1:200 and samples were incubated with primary ab for 1h at RT. a cytoplasmic signal for Carm1 was detected.

  • Applications: Western Blot, Immunofluorescence
  • Cell Tissue Type: mESC
CARM1 Antibody Western Blot,Immunofluorescence validation ( dilution) in mESC (Cat no:55246-1-AP)
FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HUVECs