CARD9 Monoclonal antibody, PBS Only

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  WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 68657-1-Ig (same clone as 68657-1-PBS)

  WB result of CARD9 antibody (68657-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CARD9 transfected THP-1 cells. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68657-1-Ig (same clone as 68657-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 68657-1-Ig (same clone as 68657-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

• 

  WB analysis of TF-1 using 68657-1-Ig (same clone as 68657-1-PBS)

  TF-1 cells were subjected to SDS PAGE followed by western blot with 68657-1-Ig (CARD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68657-1-Ig (same clone as 68657-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CARD9 antibody (68657-1-Ig, Clone: 2F3E10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68657-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CARD9 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 68657-1-PBS

Clone No.2F3E10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

hCARD9, Caspase recruitment domain-containing protein 9, 2F3E10

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Product Information

68657-1-PBS targets CARD9 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CARD9 fusion protein Ag33643
• Full Name: caspase recruitment domain family, member 9
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC008877
• Gene Symbol: CARD9
• Gene ID (NCBI): 64170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H257
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

CARD9, a member of the caspase recruitment domain (CARD) protein family, plays an important regulatory role in cell apoptosis. Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CARD9 is highly expressed in the spleen and regulates activation of NF-kappa-B by interaction with postive regulator of apoptosis BCL10 via the CARD domain.