CARD8 Polyclonal antibody

CARD8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14516-1-AP

Synonyms

DACAR, Caspase recruitment domain-containing protein 8, N-terminus, Caspase recruitment domain-containing protein 8, C-terminus, Caspase recruitment domain-containing protein 8, CARD-inhibitor of NF-kappa-B-activating ligand

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Validation Data Gallery

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  WB analysis using 14516-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14516-1-AP (CARD8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14516-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14516-1-AP (CARD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14516-1-AP

  Immunofluorescent analysis of HepG2 cells, using CARD8 antibody 14516-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: A549 cells, HUVEC cells, THP-1 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14516-1-AP targets CARD8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CARD8 fusion protein Ag5975
• Full Name: caspase recruitment domain family, member 8
• Calculated Molecular Weight: 61 kDa, 49 kDa
• Observed Molecular Weight: 61 kDa, 49 kDa
• GenBank Accession Number: BC056891
• Gene Symbol: CARD8
• Gene ID (NCBI): 22900
• RRID: AB_2068262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CARD8 belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). CARD8 may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis.