Calcyphosine Polyclonal antibody

Calcyphosine Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16926-1-AP

Synonyms

Calcyphosin, calcyphosine, CAPS, CAPS1

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Validation Data Gallery

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  WB analysis of human spleen using 16926-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 16926-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16926-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16926-1-AP (Calcyphosine antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 16926-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 16926-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 16926-1-AP (Calcyphosine antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, human spleen tissue, human colon tissue
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16926-1-AP targets Calcyphosine in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calcyphosine fusion protein Ag10330
• Full Name: calcyphosine
• Calculated Molecular Weight: 189 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC080599
• Gene Symbol: calcyphosine
• Gene ID (NCBI): 828
• RRID: AB_2070024
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

calcyphosine is a calcium-binding protein, which may play a role in the regulation of ion transport. Calcyphosine (CAPS) was initially identified in the canine thyroid but has also been detected in humans and other mammals. CAPS synthesis is increased by thyroid‐stimulating hormone and cAMP analogs that promote cell proliferation and maintain cell differentiation. Recently, CAPS was found to be involved in many kinds of tumor.