Calpain S2 Polyclonal antibody

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  WB analysis of mouse skin using 15803-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 15803-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 15803-1-AP (Calpain S2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 15803-1-AP

  IP result of anti-Calpain S2 (IP:15803-1-AP, 4ug; Detection:15803-1-AP 1:300) with mouse skin tissue lysate 2000ug.

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  IHC staining of human skin cancer using 15803-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 15803-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15803-1-AP (Calpain S2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calpain S2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15803-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

CAPNS2, CSS2, calpain, small subunit 2, Calpain small subunit 2, Calcium-dependent protease small subunit 2

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Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15803-1-AP targets Calpain S2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain S2 fusion protein Ag8534
• Full Name: calpain, small subunit 2
• Calculated Molecular Weight: 248 aa, 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005397
• Gene Symbol: Calpain S2
• Gene ID (NCBI): 84290
• RRID: AB_2259580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96L46
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain S2, also known as calpain-2, is a member of the calpain family of calcium-dependent proteases that play crucial roles in various cellular processes.