Calpain 9 Polyclonal antibody

Calpain 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17556-1-AP

Synonyms

NCL4, GC36, EC:3.4.22.-, Digestive tract-specific calpain, CAPN9

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Validation Data Gallery

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  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 17556-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 17556-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 17556-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 17556-1-AP (Calpain 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 17556-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 17556-1-AP (Calpain 9 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17556-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPN9 antibody 17556-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human stomach tissue, HL-60 cells, mouse small intestine tissue
• Positive IHC detected in: human stomach cancer tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17556-1-AP targets Calpain 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 9 fusion protein Ag11718
• Full Name: calpain 9
• Calculated Molecular Weight: 627 aa, 72 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC027993
• Gene Symbol: Calpain 9
• Gene ID (NCBI): 10753
• RRID: AB_2290833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain 9 is also named as NCL4 and belongs to the peptidase C2 family.It can be down-regulated in gastric cancer tissue and in gastric cell lines of differentiated and poorly differentiated types.It is predominantly expressed in the gastrointestinal tract and restricted to the gastric surface mucus (pit) cells in the stomach and the function of it is implicated in suppressing tumorigenesis(PMID:20686710).

Publications

Species	Application	Title
human,mouse,rat	WB,IF	Front Pharmacol; Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis; Authors - Fang Li
human	WB,IHC	Sci Rep; Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer.; Authors - Peike Peng