CAPN8 Polyclonal antibody

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  IHC staining of mouse stomach using 20971-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 20971-1-AP (CAPN8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 20971-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20971-1-AP (CAPN8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CAPN8 Polyclonal Antibody for IHC, ELISA

Cat No. 20971-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

calpain 8, CAPN8, nCL 2, NCL2, New calpain 2

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Tested Applications

• Positive IHC detected in: mouse stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20971-1-AP targets CAPN8 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calpain 8
• Calculated Molecular Weight: 79 kDa
• GenBank Accession Number: NM_001143962
• Gene Symbol: CAPN8
• Gene ID (NCBI): 388743
• RRID: AB_3085628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A6NHC0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPN8 (Calpain-8) was found to be a significant risk factor for THCA (Thyroid cancer) with a markedly elevated level of mRNA and protein in tumor tissues. This potential oncogene could induce the activation of epithelial mesenchymal transition and E2F-targeted pathways (PMID:36389799).