Calpain 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Calpain 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 11472-1-AP

Synonyms

CAPN2, Calpain M-type, Calpain M type, Calpain large polypeptide L2, Calpain 2 large subunit

---

Validation Data Gallery

• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11472-1-AP

  WB result of Calpain 2 antibody (11472-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Calpain 2 transfected HeLa cells.

• 

  WB analysis of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 (WT and CAPN2 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 11472-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11472-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11472-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 11472-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 lysates prepared and IP of CAPN2 performed using 1.0 μg of 11472-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IHC staining of human colon cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11472-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11472-1-AP (Calpain 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11472-1-AP

  Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MDA-MB-231 using 11472-1-AP

  MDA-MB-231 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 11472-1-AP at 1:300, plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

---

Tested Applications

• Positive WB detected in: A549 cells, MDA-MB-231 cells, human placenta tissue, HeLa cells, Y79 cells, C2C12 cells, mouse brain tissue, rat brain tissue, K-562 cells, PC-3 cells
• Positive IP detected in: MDA-MB-231 cells
• Positive IHC detected in: human kidney tissue, human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MDA-MB-231 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 5 publications below

Product Information

11472-1-AP targets Calpain 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Calpain 2 fusion protein Ag2029
• Full Name: calpain 2, (m/II) large subunit
• Calculated Molecular Weight: 700 aa, 80 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC021303
• Gene Symbol: CAPN2
• Gene ID (NCBI): 824
• RRID: AB_10755306
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calpain 2(Calpain-2 catalytic subunit) is also named as CANPL2, CANPml, mCANP, FLJ39928 and belongs to the peptidase C2 family. N-terminal sequencing of CAPN2 purified from human liver indicates that the N-terminal methionine is removed, resulting in a mature 699-amino acid subunit with a calculated molecular mass of 79.9 kD(PMID:2852952). It is a calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodelling and signal transduction. It has 2 isoforms produced by alternative splicing with the molecular weight of 80 kDa and 71 kDa.

Publications

Species	Application	Title
human	WB,IHC	Phytomedicine; Tiliroside disrupted iron homeostasis and induced ferroptosis via directly targeting calpain-2 in pancreatic cancer cells; Authors - Maolei Xu
	WB,IHC,IF	J Transl Med; CAPN2 promotes apalutamide resistance in metastatic hormone-sensitive prostate cancer by activating protective autophagy; Authors - Zihao Qi
mouse	WB	Pharmacol Res; Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.; Authors - Juan Wang
mouse	IF	Pharmacol Res; TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.; Authors - Wen Tian
mouse	WB	J Am Heart Assoc; Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction; Authors - Kurt D Marshall
rat	WB	Front Pharmacol; Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway.; Authors - Lei Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Wei (Verified Customer) (03-12-2020)

Weak but clear band for western blot on mouse heart lysates

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart