CAPN13 Polyclonal antibody

CAPN13 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 55371-1-AP

Synonyms

CANP 13, calpain 13

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Validation Data Gallery

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  IHC staining of mouse colon using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 55371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55371-1-AP (CAPN13 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: mouse colon tissue, human colon tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55371-1-AP targets CAPN13 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calpain 13
• Calculated Molecular Weight: 77 kDa
• GenBank Accession Number: NM_144575
• Gene Symbol: CAPN13
• Gene ID (NCBI): 92291
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit.