CAPG Polyclonal antibody

• Featured Product
• KD/KO Validated

CAPG Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 10194-1-AP

Synonyms

Actin regulatory protein CAP G, AFCP, CAPG, Macrophage capping protein, MCP

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Validation Data Gallery

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  WB analysis using 10194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 10194-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10194-1-AP (CAPG Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 10194-1-AP

  WB result of CAPG antibody (10194-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAPG transfected HeLa cells.

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  IP experiment of mouse heart using 10194-1-AP

  IP result of anti-CAPG (IP:10194-1-AP, 4ug; Detection:10194-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human prostate cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10194-1-AP (CAPG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 10194-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10194-1-AP (CAPG antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells, rat lung tissue, mouse heart, rat heart
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human prostate cancer tissue, human tonsillitis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

10194-1-AP targets CAPG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAPG fusion protein Ag0248
• Full Name: capping protein (actin filament), gelsolin-like
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 39-42 kDa
• GenBank Accession Number: BC000728
• Gene Symbol: CAPG
• Gene ID (NCBI): 822
• RRID: AB_2069179
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CapG (gelsolin-like actin-capping protein) is a member of the gelsolin superfamily proteins, which regulates actin filament length by capping or severing filaments. Studies have shown a role for CapG in regulating cell motility. The expression of CapG is upregulated under hypoxia and in turn increases the motility of cells. Recently it has been reported that the overexpression of CapG is associated with the progression of lung adenocarcinoma as well as lyphatic invasion status of cholangiocarcinoma, which may be used for prognosis of these tumors.(21908955,22155129)

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomics; Quantitative protein and mRNA profiling shows selective post-transcriptional control of protein expression by vasopressin in kidney cells.; Authors - Khositseth Sookkasem S
human	WB	J Proteome Res; Laser microdissection and two-dimensional difference gel electrophoresis reveal the role of a novel macrophage-capping protein in lymph node metastasis in gastric cancer.; Authors - Ichikawa Hiroshi H
human	WB	Clin Proteomics; Differential proteomic analysis of synovial fluid from rheumatoid arthritis and osteoarthritis patients.; Authors - Lavanya Balakrishnan
human	WB	Inflammation; PMID: 25586482; Authors - Xin Ma
human	WB	Am J Respir Cell Mol Biol; Up-regulation of two actin-associated proteins prompts pulmonary artery smooth muscle cell migration under hypoxia.; Authors - Zhang Ruifeng R ; KD Validated
human	WB	Proteomics; Proteomics-based identification of two novel direct targets of hypoxia-inducible factor-1 and their potential roles in migration/invasion of cancer cells.; Authors - Liao Shi-Hua SH