Filter: AllWBIHCIF

WB analysis using 68207-1-Ig (same clone as 68207-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
WB analysis of pig lung using 68207-1-Ig (same clone as 68207-1-PBS)
pig lung tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 68207-1-Ig (same clone as 68207-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 68207-1-Ig (CAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 68207-1-Ig (same clone as 68207-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 68207-1-Ig (same clone as 68207-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68207-1-Ig (CAP1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 68207-1-Ig (same clone as 68207-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 68207-1-Ig (same clone as 68207-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAP1 antibody (68207-1-Ig, Clone: 2H3A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68207-1-PBS in a different storage buffer formulation.

CAP1 Monoclonal antibody, PBS Only

CAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 68207-1-PBS

Clone No.2H3A10

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAP, CAP 1, CAP1, CAP1 PEN

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

68207-1-PBS targets CAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity	Human, mouse, rat, pig, rabbit
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CAP1 fusion protein Ag8876 Predict reactive species
Full Name	CAP, adenylate cyclase-associated protein 1 (yeast)
Calculated Molecular Weight	475 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC013963
Gene Symbol	CAP1
Gene ID (NCBI)	10487
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q01518
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.