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  • KD/KO Validated

CAP1 Polyclonal antibody

CAP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16231-1-AP

Synonyms

Adenylyl cyclase associated protein 1, CAP, CAP 1, CAP1, CAP1 PEN



Tested Applications

Positive WB detected inCaco-2 cells, COLO 320 cells, HepG2 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16231-1-AP targets CAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAP1 fusion protein Ag8876
Full Name CAP, adenylate cyclase-associated protein 1 (yeast)
Calculated Molecular Weight 475 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC013963
Gene Symbol CAP1
Gene ID (NCBI) 10487
RRIDAB_2172166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 52 kDa.

Protocols

Product Specific Protocols
WB protocol for CAP1 antibody 16231-1-APDownload protocol
IHC protocol for CAP1 antibody 16231-1-APDownload protocol
IP protocol for CAP1 antibody 16231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Proc Natl Acad Sci U S A

CAP1 binds and activates adenylyl cyclase in mammalian cells.

Authors - Xuefeng Zhang
humanWB,IF

J Biol Chem

Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.

Authors - Xuefeng Zhang
  • KD Validated
pigWB

Viruses

Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.

Authors - Shunli Yang
bovineWB

Cell Biochem Funct

Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.

Authors - Xiaohan Yuan
  • KD Validated
mouseWB

Cell Mol Life Sci

Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology

Authors - Anika Heinze