CAMKV Polyclonal antibody

CAMKV Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 14788-1-AP

Synonyms

VACAMKL, CaM kinase-like vesicle-associated protein



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse spleen tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14788-1-AP targets CAMKV in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMKV fusion protein Ag6438
Full Name CaM kinase-like vesicle-associated
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-75 kDa
GenBank Accession NumberBC000497
Gene Symbol CAMKV
Gene ID (NCBI) 79012
RRIDAB_2068552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMKV(CaM kinase-like vesicle-associated protein) is also named as 1G5,VACAMKL and belongs to the CAMK Ser/Thr protein kinase family. The CAMKV protein, which accumulates predominantly during postnatal development, binds to calmodulin in the presence of calcium. It is detected only in the CNS, is absent from cerebellum, but is detected in most other brain regions, especially regions of the cortex. The protein is predominantly detected in association with the plasma membrane of somatic cell and in neurites, both axons and dendrites, and is frequently observed in active zones, usually associated with vesicle-like structures. Its sequence suggests that lG5 may be membrane associated(PMID:8283228).

Protocols

Product Specific Protocols
WB protocol for CAMKV antibody 14788-1-APDownload protocol
IF protocol for CAMKV antibody 14788-1-APDownload protocol
IP protocol for CAMKV antibody 14788-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Neurodegener

Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.

Authors - Qingyang Zhang
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
human,mouseWB,IHC,IF

Ann Neurol

Paraneoplastic Calmodulin Kinase-Like Vesicle-Associated Protein (CAMKV) Autoimmune Encephalitis

Authors - Michael Gilligan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

María Inmaculada (Verified Customer) (07-15-2022)

I used the antibody for Western Blot and it worked very well. First, we used it at a 1:500 dilution first, but it was super concentrated and obtained unspecific bands. Then, I used it at a 1:2500 dilution and obtained pretty clean bands for the protein. The signal was very intense with my system, so I think I could have even diluted it a bit more.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2500
  • Cell Tissue Type: Mouse brain