CAMK2G Monoclonal antibody

CAMK2G Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C12H10

Cat no : 68519-1-Ig

Synonyms

CaMKII gamma, CAMK-II, CAMK, CaM kinase II subunit gamma, Calcium/calmodulin-dependent protein kinase type II subunit gamma

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Validation Data Gallery

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  WB analysis using 68519-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68519-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68519-1-Ig (CAMK2G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, pig brain tissue, LNCaP cells, HCT 116 cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68519-1-Ig targets CAMK2G in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAMK2G fusion protein Ag23180
• Full Name: calcium/calmodulin-dependent protein kinase II gamma
• Calculated Molecular Weight: 527 aa, 59 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC034044
• Gene Symbol: CAMK2G
• Gene ID (NCBI): 818
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.