CaMKII gamma-Specific Polyclonal antibody

CaMKII gamma-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55140-1-AP

Synonyms

CaM kinase II subunit gamma, CAMK, CAMK II, CaMK II subunit gamma, CAMK2G, CAMKG, CaMKII gamma

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Validation Data Gallery

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  WB analysis of mouse brain using 55140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 55140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55140-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55140-1-AP (CaMKII gamma-Specific antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55140-1-AP targets CaMKII gamma-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium/calmodulin-dependent protein kinase II gamma
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 45-65 kDa
• GenBank Accession Number: NM_001222
• Gene Symbol: CAMK2G
• Gene ID (NCBI): 818
• RRID: AB_10858395
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII gamma, also named as CAMKG, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. It is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CaMK2 is a Ca2+-signaling intermediate that contains α, β, γ and δ subunits. The antibody is specific to CAMK2G.

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma.; Authors - Ya Peng
human	WB	Biomed Pharmacother; Novel synthetic tosyl chloride-berbamine regresses lethal MYC-positive leukemia by targeting CaMKIIγ/Myc axis.; Authors - Qingfeng Yu