CaMKII delta-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

CaMKII delta-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 20667-1-AP

Synonyms

CaM kinase II subunit delta, CaMK II subunit delta, CAMK2D, CAMKD, CaMKII delta-Specific

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Validation Data Gallery

• 

  WB analysis using 20667-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 20667-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20667-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20667-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20667-1-AP (CaMKII delta-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 20667-1-AP

  IP result of anti-CaMKII delta-Specific (IP:20667-1-AP, 4ug; Detection:20667-1-AP 1:600) with rat brain tissue lysate 4000ug.

• 

  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 20667-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20667-1-AP (CaMKII delta-Specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse skeletal muscle tissue, mouse heart tissue, human skeletal muscle tissue, rat brain tissue
• Positive IP detected in: rat brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

20667-1-AP targets CaMKII delta-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: calcium/calmodulin-dependent protein kinase II delta
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_001221
• Gene Symbol: CAMK2D
• Gene ID (NCBI): 817
• RRID: AB_10695498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CaMKII delta, also named as CAMKD and CaMKII delta, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and CaMK subfamily. CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release, composed of four different chains: alpha, beta, gamma, and delta. The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. The different chains assemble into homo-or heteromultimeric holoenzymes composed of 8 to 12 subunits. This antibody is specific to the C-term of delta chain, CaMKII delta. It can recognize most isoforms the gene encodes, from 50-72kd. It can recognize the isoform 2,3,4,9,11 of CAMK2D.

Publications

Species	Application	Title
human	WB	Cell Death Differ; CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma; Authors - You Heng Chuah
human	WB	Mol Ther Nucleic Acids; circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.; Authors - Cui Ma
mouse	IHC	Cell Death Dis; miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.; Authors - Xudong Zhang
human	WB	Reprod Biol Endocrinol; In vitro fertilization with frozen embryo transfer increased histamine-mediated contractile sensitivity via PKCβ in human umbilical vein; Authors - Jiaqi Tang
rat	WB	J Pain Res; Proteomic Analysis of Emodin Treatment in Neuropathic Pain Reveals Dysfunction of the Calcium Signaling Pathway.; Authors - Peng Chen
human	WB	Oncol Lett; PMID: 30127991; Authors - Xiaoli Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jonathan (Verified Customer) (11-11-2019)

Works well for WB (1:1000) and IF (1:200)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:200