CAMK1D Polyclonal antibody

• Featured Product
• KD/KO Validated

CAMK1D Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13613-1-AP

Synonyms

CaM K1, CaM KI delta, CaM kinase I delta, CaM kinase ID, CAMK1D, CaMKI delta, CaMKI like protein kinase, CaMKID, CKLiK

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Validation Data Gallery

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  WB analysis of HeLa using 13613-1-AP

  WB result of CAMK1D antibody (13613-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMK1D transfected HeLa cells.

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  WB analysis using 13613-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13613-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 13613-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13613-1-AP (CAMK1D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 13613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13613-1-AP (CAMK1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, HeLa cells, mouse thymus tissue, rat pancreas tissue
• Positive IHC detected in: mouse brain tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13613-1-AP targets CAMK1D in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMK1D fusion protein Ag4517
• Full Name: calcium/calmodulin-dependent protein kinase ID
• Calculated Molecular Weight: 357 aa, 40 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC035745
• Gene Symbol: CAMK1D
• Gene ID (NCBI): 57118
• RRID: AB_2228498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.