CALML5 Polyclonal antibody

CALML5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13059-1-AP

Synonyms

CALML5, calmodulin like 5, Calmodulin like protein 5, Calmodulin like skin protein, CLSP

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Validation Data Gallery

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  IHC staining of human skin cancer using 13059-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 13059-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13059-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13059-1-AP (CALML5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human skin cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

13059-1-AP targets CALML5 in IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALML5 fusion protein Ag3734
• Full Name: calmodulin-like 5
• Calculated Molecular Weight: 146 aa, 16 kDa
• GenBank Accession Number: BC039172
• Gene Symbol: CALML5
• Gene ID (NCBI): 51806
• RRID: AB_2069463
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CALML5, also known as calmodulin-like skin protein (CLSP), a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.

Publications

Species	Application	Title
human	IHC,IF	Ann Diagn Pathol; Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia.; Authors - Abdulmajeed Ahmad A AA
human	IHC	Elife; Dalpiciclib partially abrogates ER signaling activation induced by pyrotinib in HER2+HR+ breast cancer; Authors - Jiawen Bu