CALML3 Polyclonal antibody

CALML3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17275-1-AP

Synonyms

CALML3, calmodulin like 3, Calmodulin like protein 3, CaM like protein, CLP

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Validation Data Gallery

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  WB analysis using 17275-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17275-1-AP (CALML3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 17275-1-AP

  IP result of anti-CALML3 (IP:17275-1-AP, 4ug; Detection:17275-1-AP 1:300) with A431 cells lysate 2400 ug.

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  IHC staining of human liver cancer using 17275-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17275-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17275-1-AP (CALML3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 17275-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CALML3 antibody (17275-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17275-1-AP targets CALML3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALML3 fusion protein Ag11037
• Full Name: calmodulin-like 3
• Calculated Molecular Weight: 149 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC031889
• Gene Symbol: CALML3
• Gene ID (NCBI): 810
• RRID: AB_2878374
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.