CALHM2 Polyclonal antibody, PBS Only

CALHM2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19931-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 19931-1-AP (same clone as 19931-1-PBS)

  Mouse brain extracted membrane protein and total protein lysates were subjected to SDS PAGE followed by western blot with 19931-1-AP (CALHM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 19931-1-AP (same clone as 19931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19931-1-AP (same clone as 19931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19931-1-AP (same clone as 19931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 19931-1-AP (same clone as 19931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 19931-1-AP (CALHM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 19931-1-AP (same clone as 19931-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CALHM2 antibody (19931-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19931-1-PBS in a different storage buffer formulation.

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Product Information

19931-1-PBS targets CALHM2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALHM2 fusion protein Ag13909
• Full Name: calcium homeostasis modulator 2
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 32-36 kDa
• GenBank Accession Number: BC000039
• Gene Symbol: CALHM2
• Gene ID (NCBI): 51063
• RRID: AB_2878626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CALHM2, also named as FAM26B, is a pore-forming subunit of a voltage-gated ion channel. It's a critical ATP-releasing channel that modulates neural activity and as a potential risk factor of depression. There're three isoforms of CALHM2 with calculated MW 36 kDa, 24 kDa and 22 kDa. It's about 32-36 kDa in western blot with membrane protein extraceted lysate.