CALCOCO1 Recombinant antibody

CALCOCO1 Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241127G7

Cat no : 84009-4-RR

Synonyms

PP13275, Coiled-coil coactivator protein, Cocoa, Calphoglin, Calcium-binding and coiled-coil domain-containing protein 1

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Validation Data Gallery

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  WB analysis using 84009-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84009-4-RR (CALCOCO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 84009-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84009-4-RR (CALCOCO1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, MCF-7 cells, HeLa cells, HEK-293T cells, Ramos cells, rat brain tissue
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84009-4-RR is CALCOCO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CALCOCO1 fusion protein Ag13876
• Full Name: calcium binding and coiled-coil domain 1
• Calculated Molecular Weight: 691 aa, 77 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC003177
• Gene Symbol: CALCOCO1
• Gene ID (NCBI): 57658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.