CALCA/CGRP Monoclonal antibody, PBS Only (Capture)

CALCA/CGRP Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1A12A1

Cat no : 68774-1-PBS

Synonyms

Calcitonin, CALCA, CALC1, Alpha-type CGRP, Alpha type CGRP

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Validation Data Gallery

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  WB analysis of TT using 68774-1-Ig (same clone as 68774-1-PBS)

  TT cells were subjected to SDS PAGE followed by western blot with 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

• 

  Sample test of MP50293-1

  Cytometric bead array in cell lysate using MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Cell lysate: TT, HepG2.

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  IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 68774-1-Ig (same clone as 68774-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68774-1-Ig (CALCA/CGRP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68774-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP50293-1

  Cytometric bead array standard curve of MP50293-1, CALCA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68774-1-PBS. Detection antibody: 68774-2-PBS. Standard:Ag26141. Range: 0.781-100 ng/mL.

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Product Information

68774-1-PBS targets CALCA/CGRP as part of a matched antibody pair:

MP50293-1: 68774-1-PBS capture and 68774-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CALCA/CGRP fusion protein Ag26141
• Full Name: calcitonin-related polypeptide alpha
• Calculated Molecular Weight: 141 aa, 15 kDa
• GenBank Accession Number: BC093753
• Gene Symbol: CGRP
• Gene ID (NCBI): 796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.