CALCA/CGRP Polyclonal antibody

CALCA/CGRP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22244-1-AP

Synonyms

Alpha type CGRP, CALC1, CALCA, Calcitonin, CGRP, CGRP I, CGRP1, CT, KC, Procalcitonin

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Validation Data Gallery

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  WB analysis using 22244-1-AP

  TT cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 22244-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22244-1-AP (CGRP antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of TT using 22244-1-AP

  IP result of anti-CALCA/CGRP (IP:22244-1-AP, 4ug; Detection:22244-1-AP 1:600) with TT cells lysate 945 ug.

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Tested Applications

• Positive WB detected in: TT cells, Transfected HEK-293 cells
• Positive IP detected in: TT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22244-1-AP targets CALCA/CGRP in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CALCA/CGRP fusion protein Ag17737
• Full Name: calcitonin-related polypeptide alpha
• Calculated Molecular Weight: 141 aa, 15 kDa
• Observed Molecular Weight: 14 kDa, 5 kDa
• GenBank Accession Number: BC093753
• Gene Symbol: CGRP
• Gene ID (NCBI): 796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).