CALCA/CGRP Polyclonal antibody

CALCA/CGRP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22244-1-AP

Synonyms

Alpha type CGRP, CALC1, CALCA, Calcitonin, CGRP, CGRP I, CGRP1, CT, KC, Procalcitonin



Tested Applications

Positive WB detected inTT cells, Transfected HEK-293 cells
Positive IP detected inTT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22244-1-AP targets CALCA/CGRP in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CALCA/CGRP fusion protein Ag17737
Full Name calcitonin-related polypeptide alpha
Calculated Molecular Weight 141 aa, 15 kDa
Observed Molecular Weight14 kDa, 5 kDa
GenBank Accession NumberBC093753
Gene Symbol CGRP
Gene ID (NCBI) 796
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Calcitonin gene-related peptide (CGRP) is a 37-amino acid neuropeptide, which is primarily localized to C and Aδ sensory fibers. These fibers display a wide innervation throughout the body, with extensive perivascular localization, and have a dual role in sensory (nociceptive) and efferent (effector) function. Discovered 30 years ago, it is produced as a consequence of alternative RNA processing of the calcitonin gene. CGRP has two major forms (α and β).

Protocols

Product Specific Protocols
WB protocol for CALCA/CGRP antibody 22244-1-APDownload protocol
IP protocol for CALCA/CGRP antibody 22244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols