CADM3 Polyclonal antibody

CADM3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15660-1-AP

Synonyms

BIgR, Brain immunoglobulin receptor, CADM3, cell adhesion molecule 3, IGSF4B, Necl 1, NECL1, Nectin like protein 1, synCAM3, TSLC1 like protein 1, TSLL1

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Validation Data Gallery

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  WB analysis using 15660-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15660-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 15660-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15660-1-AP (CADM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, Jurkat cells, SH-SY5Y cells, mouse cerebellum tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15660-1-AP targets CADM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CADM3 fusion protein Ag8081
• Full Name: cell adhesion molecule 3
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38-50 kDa
• GenBank Accession Number: BC033819
• Gene Symbol: CADM3
• Gene ID (NCBI): 57863
• RRID: AB_2068044
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CADM3, also known as IGSF4B or TSLL1, is a transmembrane protein that is involved in the cell-cell adhesion. CADM3 is mainly expressed in the nervous system and plays a role in the formation of synapses, axon bundles, myelinated axons and cerebellar morphogenesis (PMID: 18420026).

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