CADM1 Polyclonal antibody

CADM1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14335-1-AP

Synonyms

NECL-2, NECL2, Necl 2, IGSF4A, IgSF4

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Validation Data Gallery

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  WB analysis of mouse liver using 14335-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14335-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14335-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14335-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, A549 cells, human placenta tissue, mouse lung tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below

Product Information

14335-1-AP targets CADM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CADM1 fusion protein Ag5621
• Full Name: cell adhesion molecule 1
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-60 kDa
• GenBank Accession Number: BC047021
• Gene Symbol: CADM1
• Gene ID (NCBI): 23705
• RRID: AB_2243801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CADM1 (cell adhesion molecule 1), also referred to as SgIGSF, Necl-2 or TSLC-1, is a cell adhesion molecule belonging to the immunoglobulin superfamily. It can mediate homophilic and heterophilic cell-cell adhesion. CADM1 is expressed by a variety of cells including neurons, mast cells, spermatogonia, pancreatic islet cells, epithelial cells such as lung alveolar cells and biliary epithelial cells. CADM1 is involved in carcinoma pathogenesis and the expression of CADM1 is frequently down-regulated in various tumors derived from epithelial cells. It acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. (PMID: 18332875; 17326163; 20340131)

Publications

Species	Application	Title
mouse	WB	PLoS One; Necl-4/SynCAM-4 is expressed in myelinating oligodendrocytes but not required for axonal myelination.; Authors - Zhu Ying Y
human	WB	Onco Targets Ther; miR-424-5p Promotes Proliferation, Migration and Invasion of Laryngeal Squamous Cell Carcinoma.; Authors - Yujun Li
human	WB	Oncol Lett; miR-214-3p promotes the proliferation, migration and invasion of osteosarcoma cells by targeting CADM1.; Authors - Haiqing Cai
human	WB	BMC Cancer; CADM1 impairs the effect of miR-1246 on promoting cell cycle progression in chemo-resistant leukemia cells; Authors - Bei Xie
	WB	Fish Shellfish Immunol; Mucous cell histopathology and label-free quantitative proteomic analysis of skin mucus in fat greenling (Hexagrammos otakii) infected with Vibrio harveyi; Authors - Xiaoyan Wei
human,mouse	WB	Adv Healthc Mater; Nanointegrative Glycoengineering-Activated Necroptosis of Triple Negative Breast Cancer Stem Cells Enables Self-Amplifiable Immunotherapy for Systemic Tumor Rejection; Authors - Youbo Zhao

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